Fluorophos ALP Diagnostic Test – Substrate Test 1b
Low readings suggest incomplete mixing of substrate. 1/ Check buffer has been mixed with substrate. Un-reconstituted substrate will account for low readings on positive samples. 2/ Mix a new batch of substrate following the instructions provided in the pack. 3/ Pipette 2ml of substrate into a cuvette and warm. 4/ With the instrument in A/D mode place the cuvette into the well.