Alivion Spark M-20 mobile methanol control

ATMOS Pipette Accuracy Tester

  • High accuracy
  • Results in less than 30 seconds
  • Leak measurement
  • No calibration required

Air-displacement pipettes need to be checked at regular intervals in order to comply with the accuracy standards. The calibration verification normally requires very careful weighting with a laboratory scale. With ATMOS, you can check the accuracy of your pipettes daily, which allows a continuous performance monitoring between the usual calibration procedures.

With ATMOS you can test single channel and 8 channel pipettes in less than 30 seconds, without the need for a balance and manipulating liquids.

ATMOS measures, in dry conditions, the exact displacement volume as well as performing a leak detection. The measurement data is stored in the instrument and can be downloaded to a computer for pipette performance monitoring.

Test Procedure

Testing pipettes with the ATMOS takes just 4 simple steps:

  1. Select test volume
  2. Place the tip on ATMOS
  3. Press and release the pipette piston (10 sec. cycle)
  4. Read the dispensed volume and the leak on ATMOS screen

ATMOS does not need calibrating. It measures the volume dispensed by the pipette by comparing with an internal fixed volume metal cavity. This reference volume is calibrated at the factory within 0.1% accuracy.

Can I use ATMOS to calibrate my pipettes?

The best use of ATMOS is the daily monitoring of the accuracy of your pipettes. It will detect any change of calibration that may, for example, be related to a shock (fall on the floor) or forcing the dial beyond the limit. This allows you to put damaged pipette on hold and not use them until your next calibration by gravimetry. It is also possible to use ATMOS to adjust a pipette in case of shift of accuracy (most pipettes have a special tool to adjust the volume). This is much easier than gravimetry.

What is the leak measurement?

ATMOS measures the air leak of the pipette or of the tip during its function. High leakage can be related to a damaged piston in the pipette or to a defect in connection of the disposal tip. The leak value is given in percentage of volume loss per second. A leak of less than 1% is acceptable. The leak affects the accuracy of the pipette, especially when using viscous liquids. Therefore, a pipette with a leak should be serviced (grease the piston and check tip cone connection). ATMOS will indicate wrong results when the pipette is leaky.

How does ATMOS accuracy compare to gravimetry method?

The random error of ATMOS readings are similar to those observed in gravimetry measurement with a laboratory scale. The absolute accuracy of ATMOS stays within ± 1% ( ± 0.1 µl for volumes below 10µl ). Since ATMOS measures the true air displacement of the piston in the pipette, the equivalent liquid volume is calculated using an algorithm for compensation of negative pressure that is slightly lowering the effective volume of aspirated liquid. The compensation algorithm is based on the use of standard disposable tips. When calibrating by gravimetry your pipettes with special tips, such as long tips, ATMOS reading value may differ.

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