1/ Collect a homogenous sample and centrifuge. Re-suspend the sample using Buffer A. 2/ Transfer 5 ┬Ál of the re-suspended sample into the PCR reagent tube. Place the tube into the Thermocycler and run the program.
   
 
3/ Remove the PCR tube from the Thermocycler and add Buffer B. Dispense the PCR tube contents onto the test cassette window. Wait 3 minutes and retract the test cassette switch to reveal the test results. One line indicates negative result, two lines indicates semi-quantitative positive results.