1/ After 18 hours enrichment, transfer 500 μl sample to the 1.5 ml tube. Heat the sample for 10 minutes, then allow to cool to room temperature. 2/ Transfer 5 μl of heat inactivated sample into the reagent tube. Place the tube into the thermocycler and run the program.
   
 
3/ Remove the reagent tube from the thermocycler. Dispense the reagent tube contents onto the test cassette window. Wait 3 minutes and retract the test cassette to reveal the test results. One line indicates a negative result, two lines indicate a positive result.