1/ Collect a homogenous sample and centrifuge. Re-suspend the sample using the Buffer A. 2/ Transfer resuspended sample into provided Digest reagent tube. Place tube into Thermocycler and run program.
   
3/ Transfer sample from Digest reagent tube into provided PCR reagent tube. Place tube into Thermocycler and run program.. 4/ Remove PCR Tube from Thermocycler and add proprietary Buffer B. Dispense PCR Tube contents onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results. Use the Signal Quantification Card or Veriflow Reader for precise quantification.