When the Fluorophos is switched on allow 2 hours before use to ensure complete electronic stabilization.
1/ Pour the entire contents of one of the substrate buffer bottle into one of the powdered Fluorophos substrate bottle.
2/ Mix by gentle inversion for 3 minutes, to dissolve all of the substrate. Allow to stand for 15 minutes.
3/ Note date of mixing on bottle. The shelf life will be 60 days, stored at 2 – 6oC. To check the substrate warm 2 ml and place in Fluorophos. Run A/D Test (In Setup). Stabilized result should be less than 1200 (If over, replace substrate). If degrading quickly, contact QCL for advice.
1/ Dispense 2ml of prepared Fluorophos substrate into a sufficient number of cuvettes for the proposed tests.
2/ Place substrate cuvettes in the block heater for a minimum of 10 minutes.
3/ Select product to be tested from the Fluorophos menu. Press TEST, ENTER, pick channel, ENTER, enter ID number if necessary, ENTER.
4/ Pipette 75ul of the milk/cream sample, into a pre-warmed substrate cuvette (see Pipetting Guide for more details).
5/ Press ‘TEST’ and select the ‘ALP Dairy’ menu.
6/ Scroll through the menu and press ‘Enter’ when the product to be tested is displayed.
7/ Pipette 75ul of the milk/cream sample, into a pre-warmed substrate cuvette.
8/ Mix immediately then insert the cuvette in the Fluorophos, within 20 seconds, and press ‘START’ to measure the sample.
9/ The sample is conditioned/heated for 60 seconds. 10/ After a further 2 minutes the result is presented in mU/L.